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Interpretation of Western Blot Assay in Serodiagnosis of HIV-1 Infection

Purpose:

To use Western blot testing to help confirm a positive screening test for HIV antibodies.

Specialty:

Infectious Diseases

Objective:

laboratory tests

ICD-10:

B24

Description:

The Western Blot assay for detection of anti-HIV-1 antibodies is used as a confirmatory test for positive ELISA assays for HIV-1. It is not appropriate to use the Western blot assay as a screening test for HIV-1 antibodies, largely due to the number of indeterminate results.

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