Transketolase can be used as a functional test for thiamine.
Specimen: Whole blood is collected with heparin as anticoagulant. An erythrocyte hemolysate is prepared, which is stable for at least 4 weeks if frozen.
Analysis:
(1) NADH dependent Erythrocyte Transketolase, measured at 340 nm at 37 C.
(2) If anemia is present, erythrocyte transketolase activity should be expressed per unit hemoglobin.
Interpretation:
• normal is 0.75-1.30 IU transketolase per gram hemoglobin
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