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The Lineweaver-Burk Plot for an Enzyme-Catalyzed Reaction

Description

The Lineweaver-Burk plot is a means of expressing the substrate and reaction velocity data from an enzyme-catalyzed reaction as a straight line. The x- and y- axis intercepts can be used t identify the maximum velocity (Vmax) and Km for the reaction system.


 

Plot:

• x axis: 1 / (substrate concentration)

• y axis: 1 / (reaction velocity)

• This data will have a positive slope and will be in the upper right quadrant of the graph space.

• The data is then extrapolated into a line equation for y = mx + b and extended to where y = 0.

 

EXCEL functions

• slope: INDEX (LINEST (array for Y values, array for X values),1)

• y-axis intercept : INDEX (LINEST (array for Y values, array for X values), 2)

 

x-axis intercept =

= ((-1) * (y-axis intercept)) / (slope for line)

 

maximum velocity (Vmax) =

=1 / (y-axis intercept)

 

Km =

= (-1) * (1 / (x-axis intercept))

 


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