Subgroups of Iqbal et al in Alzheimer's Disease Associated with CSF Levels of Ubiquitin, beta-Amyloid(1-42) and Tau Protein

Description

Iqbal et al measured the levels of ubiquitin, beta-amyloid(1-42) and tau protein in the cerebrospinal fluid (CSF) of patients with Alzheimer's Disease. They were able to identify 5 separate patterns of these proteins. The authors are from New York State Institute for Basic Research in Developmental Disabilities, University of Kuopio (Finland), Sahlgren's University Hospital (Molndal), Karolinska Institute and Innogenetics NV.

Specimen: cerebrospinal fluid

Analysis:

(1) ubiquitin: competitive inhibition ELISA using monoclonal antibody 5-25 (Signet Labs)

(2) beta-Amyloid(1-42): sandwich ELISA (Innogenetics)

(3) tau protein: sandwich ELISA (Innogenetics)

Ubiquitin	Beta Amyloid	Tau	Pattern
>= 500 ng/mL	NA	NA	ATURO
< 500 ng/mL	>= 900 pg/mL	NA	HARO
< 500 ng/mL	< 900 pg/mL	>= 920 pg/mL	ATEO
< 500 ng/mL	< 900 pg/mL	520 to 919 pg/mL	AELO
< 500 ng/mL	< 900 pg/mL	< 520 pg/mL	LEBALO

Pattern	Disease Onset	Descriptor
ATURO	recent	high ubiquitin, low beta-amyloid, high tau
HARO	recent	high beta-amyloid
ATEO	early	low beta-amyloid, high tau
AELO	late	low beta-amyloid, high incidence APOE4
LEBALO	late	low beta-amyloid, high incidence of Lewy bodies

Limitation:

• The performance of the assays would have to be similar to that of the authors to get the same separations.

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Subgroups of Iqbal et al in Alzheimer's Disease Associated with CSF Levels of Ubiquitin, beta-Amyloid(1-42) and Tau Protein

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