Lin et al identified multiple occurrence of false-positive PCR results while testing for norovirus. Problems can occur when a definitive diagnosis is made based on the results of a single test. The authors are from Chang Gung University, Chang Gung Memorial Hospital and Children's Hospital Taoyuan in Taiwan.
(1) positive stool culture for pathogenic bacteria
(2) parenteral antibiotic therapy
The false-positive result was due to amplification of DNA from the patient. This showed the same molecular length as the norovirus target. This was determined by sequencing the material.
A confirmatory test should be performed on a specimen with a positive PCR test for RdRp, especially if the clinical picture suggests another diagnosis. A stool culture should also be performed.
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