Parameters:
(1) target dose of CD34+ cells per kilogram body weight
(2) body weight of the patient
(3) number of CD34+ cells in peripheral blood
(4) collection efficiency of CD34+ cells
(5) total volume of blood that can be processed during an apheresis session
total dose of CD34+ cells =
= (body weight) * (target dose in CD34+ cells per kilogram)
where:
• The target dose used by Ford et al was 5 * 10^6 per kilogram
• I assume the ideal body weight should be used for dosing since that corresponds to blood volume.
total amount of blood to process in mL =
= (total dose of CD34+ cells) / ((CD34+ cell count per µL) * 1000 * (efficiency as a decimal fraction))
number of apheresis sessions required =
= (total amount of blood to process in mL) / (total volume of blood that can be processed in 1 apheresis session)
where:
• The total volume of blood that can be processed is usually a multiple of the patient’s blood volume, often 2.5 times. The patient’s blood volume can be calculated in Chapter 02.
