Papain is a protease derived from the papaya. It is a cysteine protease that cleaves peptide bonds of basic amino acids, glycine or leucine. It also hydrolyzes esters and amides.
Molecular weight: 21,000 Daltons
(1) units of activity: 1 unit is capable of hydrolyzing 1 micromole of N-benzoyl-L-arginine ethyl ester (BAEE) per minute at 25°C and pH 6.2
(2) units per mg protein
(1) preparation of Fab antibody fragments
(2) cell dissociation
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