The net negative charge associated with a serum protein can be estimated based on its concentration and physiochemical features.
net negative charge in mEq/L =
= (serum concentration in g/L) * (buffer value for the protein) * ((pH) – (isoelectric point for the protein))
• The buffer value for most serum proteins is 0.1 mmol per g per unit pH
• A total serum protein of 7 g/dL at body temperature and pH of 7.4 would have a net negative charge of 12-16 mEq/L.
from Geigy Scientific Tables, Seventh Edition, page 580.
To read more or access our algorithms and calculators, please log in or register.
Specialty: Endocrinology, Clinical Laboratory, Emergency Medicine, Critical Care