Molecular Classification of Zhang et al for Infiltrating Ductal Carcinoma Based on Immunohistochemical Staining

Description

Zhang et al classified infiltrating ductal carcinomas into molecular types based on immunohistochemical staining. The authors are from Sichuan University, RTI Health Solutions (Triangle Park, NC), Biocare Inc (Concord, Ca), University of Rochester, Luzhou Medical College and the Japan-China Friendship Hospital in Beijing.

Patient selection: infiltrating ductal carcinoma

Parameters:

(1) estrogen receptor (ER)

(2) progesterone receptor (PR)

(3) HER2/neu

(4) Ki-67 index

(5) EGFR

(6) CK5/6

ER and PR	HER2/neu	Ki-67	EGFR and CK5/6	Type
one or both positive	negative	< 15% (negative)	NA	luminal A
one or both positive	negative	>= 15% (positive)	NA	luminal B
one or both positive	positive	NA	NA	luminal HER2/neu
both negative	positive	NA	NA	HER/neu
both negative	negative	NA	one or both positive	basal-like
both negative	negative	NA	one or both negative	unclassified

Antibodies	Clone	Manufacturer	Preparation
ER	ID5 and ER-2-123	DAKO	ready to use
PR	PgR 1294	DAKO	ready to use
HER2/neu	polyclonal	DAKO	ready to use
Ki-67	MIB-1	DAKO	dilute 1:100
EGFR	2-18C9	DAKO	ready to use
CK5/6	D5/16B4	DAKO	dilute 1 to 50

Antibodies	Staining	Positive If
ER	nuclear	Allred score >= 3
PR	nuclear	Allred score >= 3
HER2/neu	membrane	>= 30% with 3+ strong
Ki-67	nuclear	>= 15% any intensity
EGFR	membrane	>= 10% of 3+
CK5/6	cytoplasmic	>= 10% of strong

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Description

Specialty

ICD-10