Masshoff et al estimated the muscle mass of the heart from its specific gravity.



(1) total heart weight

(2) total heart volume


specific gravity of the heart =

= (total heart weight in grams) / (total heart volume in mL)


The percent of heart weight that is cardiac muscle can be estimated from the specific gravity. This ranges from 100% for a specific gravity of 1.055 to 0% for a specific gravity of 0.941.


percent of heart weight that is muscle =

= ((8.77193 * (specific gravity)) - 8.254386) * 100*



• The preparation of the heart must be meticulous and standardized for reproducible results. Even a small amount of water or blood can affect the result.

• The weight and volume must be taken meticulously.


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