Proteins in solution absorb ultraviolet light at 280 nm primarily due to the presence of tryptophan, tyrosine and phenylalanine.
Light source: ultraviolet
Parameters:
(1) absorption at 280 nm from 0 to 1.0
(2) absorption at 260 nm from 0 to 1.0 (which removes interference from nucleic acids)
protein concentration in mg/mL =
= (1.55 * (absorption at 280 nm)) - (0.76 * (absorption at 260 nm))
Interpretation:
• The applicable protein concentration range is <= 1.5 mg/dL.
• The absorbance may be affected by pH, ionic strength and the presence of other chemicals.
To read more or access our algorithms and calculators, please log in or register.
Specialty: Clinical Laboratory
