Ring et al developed a panel of 5 immunohistochemical stains that can distinguish between various types of non-small cell lung carcinoma. The authors are from the Comprehensive Cancer Center of Huntsville, the University of Alabama, University Hospital Zurich and Applied Genomics, Inc.
Patient selection: primary non-small cell lung carcinoma (NSCLC)
Parameters:
(1) cytoplasmic and/or membrane staining with CEACAM5 (carcinoembryonic anigen-related cell adhesion molecular 5)
(2) plasma membrane staining with SLC7A5 (solute carrier family 7, member 5)
(3) cytoplasmic staining with TRIM29 (tripartite motif containing 29)
(4) cytoplasmic staining with CK5/6 (cytokeratin 5/6)
(5) staining with MUC1 (mucin 1, cell surface associated)
|
Parameter |
Findings |
Points |
|
staining with CEACAM5 |
<= 10% of invasive tumor cells |
0 |
|
|
> 10% of invasive tumor cells |
1 |
|
staining with SLC7A5 |
<= 10% of invasive tumor cells |
0 |
|
|
> 10% of invasive tumor cells |
1 |
|
staining with TRIM29 |
<= 10% of invasive tumor cells |
0 |
|
|
> 10% of invasive tumor cells |
1 |
|
staining with CK5/6 |
<= 10% of invasive tumor cells |
0 |
|
|
> 10% of invasive tumor cells |
1 |
|
staining with MUC1 |
<= 10% of invasive tumor cells |
0 |
|
|
> 10% of invasive tumor cells |
1 |
total immunohistochemical score =
= (1.25 * (points for SLC7A5)) - (1.43 * (points for CEACAM5)) + (2.2 * (points for TRIM29)) - (1.07 * (points for MUC1)) + (2.27 * (points for CK5/6)) - 0.95
Interpretation:
• minimum score: -3.45
• maximum score: 4.77
• There are 32 possible score combinations.
|
Immunohistochemical Score |
Interpretation |
|
< - 0.95 |
adenocarcinoma |
|
-0.95 to 0.30 |
unclassified |
|
> 0.30 |
squamous cell carcinoma |
where:
• If the (-0.95) is removed from the equation then any tumor < 0 is an adenocarcinoma and anything > 1.25 is a squamous cell carcinoma.
Specialty: Hematology Oncology, Surgery, general, Pulmonology
ICD-10: ,
