Subgroups of B substance occur rarely.
(1) weak or absent reaction with antisera (which now occurs infrequently with use of oligoclonal antisera, which are able to agglutinate cells with decreased or variant B antigens)
Method for evaluation:
(1) reaction of cells with specific antisera or with lectins having an activity mimicing an antisera
(2) if the person is a secretor, the presence or absence of A substance in the saliva
agglutination of cells with anti-B
1+, mixed field
0 to +/-
agglutination of cells with anti-A,B
2+ mixed field
0 to 2+
agglutination of cells with anti-A
agglutination of cells with anti-H or lectin Eulex europaeus
B substance in saliva of secretors
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Specialty: Clinical Laboratory