The length of time required for a probed to hybridize with its target sequence is affected by the probe characteristics, its concentration, and the reaction conditions.
• Probe complexity is length of any sequence repeated to make up the completed probe; if there are no repeated sequence, then the probe complexity equals the probe length; if the probe consists of n repeats of a nucleotide sequence with m nucleotides, then probe complexity equals m and probe length equals (m * n)
• 3.5 * (10 ^5) is the nucleation constant for a sodium concentration of 0.4 - 1.0 M, a hybridization temperature 25°C below the Tm for the reaction, and a pH between 5-9
• The probe concentration in solution is in moles of probe molecules per liter.
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