McCarty et al described a histochemical score that could be used to quantitate the intensity of staining shown by an immunohistochemical tumor marker.
For a histochemical marker, each of the following is recorded:
(1) the intensity of staining from 0 to 4+
(2) the percent of the tumor showing each staining intensity
|
Staining Intensity |
Factor (x + 1) |
Value |
|---|---|---|
|
0 (none) |
NA |
0 * (percent of tumor cells negative) |
|
1+ (weak) |
2 |
2 * (percent of tumor staining 1+) |
|
2+ |
3 |
3 * (percent of tumor staining 2+) |
|
3+ |
4 |
4 * (percent of tumor staining 3+) |
|
4+ (strong) |
5 |
5 * (percent of tumor staining 4+) |
where:
• The percent staining is a number from 0 to 100.
• The sum of all the percent staining must equal 100.
• The factor for each level is (x + 1), where x indicates the staining intensity (x+)
histochemical score =
= SUM(values for each level of positive staining)
Interpretation
• minimum score: 0
• maximum score: 500
• For each tumor marker the lowest score determining a positive reaction needs to be determined.
Purpose: To calculate the histochemical score (Hscore) for an immunohistochemical tumor marker.
Specialty: Hematology Oncology, Surgery, general, Clinical Laboratory
Objective: risk factors, laboratory tests, severity, prognosis, stage
ICD-10: C00-C14, C15-C26, C30-C39, C40-C41, C43-C44, C45-C49, C50-C50, C51-C58, C60-C63, C64-C68, C69-C72, C73-C75, C76-C80, C81-C96, C97-C97,
