Description

HBSS (Hank's Balanced Salt Solution) is an important solution for handling cells and tissues since it maintains the proper osmotic and pH balance.


 

Component

Molecular Weight

NaCl

58.44

KCl

74.55

CaCl2

110.98

MgSO4•7H2O

246.47

MgCl2•6H2O

203.303

Na2HPO4•2H2O

177.99

KH2PO4

136.086

D-glucose

180.156

NaHCO3

84.01

 

 

Component

Grams per liter

Molarity

NaCl

8

0.14

KCl

0.400

0.005

CaCl2

0.140

0.001

MgSO4•7H2O

0.100

0.0004

MgCl2•6H2O

0.100

0.0005

Na2HPO4•2H2O

0.060

0.0003

KH2PO4

0.060

0.0004

D-glucose

1

0.006

NaHCO3

0.350

0.004

 

A pH indicator may be added, resulting in a colored solution.

Component

Molecular Weight

NaCl

58.44

KCl

74.55

CaCl2

110.98

MgSO4•7H2O

246.47

MgCl2•6H2O

203.303

Na2HPO4•2H2O

177.99

KH2PO4

136.086

D-glucose

180.156

NaHCO3

84.01

 

 

Component

Grams per liter

Molarity

NaCl

8

0.14

KCl

0.400

0.005

CaCl2

0.140

0.001

MgSO4•7H2O

0.100

0.0004

MgCl2•6H2O

0.100

0.0005

Na2HPO4•2H2O

0.060

0.0003

KH2PO4

0.060

0.0004

D-glucose

1

0.006

NaHCO3

0.350

0.004

 

A pH indicator may be added, resulting in a colored solution.


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