Troubleshooting Overstaining with Eosin

Are you troubleshooting an H&E stain with cytoplasmic staining that is too dark?

Is the eosin solution formulated in alcohol?

Is the concentration of the eosin too high?

Does the eosin stain also contains phloxine?

Was the slide in the eosin solution for too long?

Was the rinsing in alcohol after the eosin step adequate?

Was an alcohol other than ethanol to rinse the slide after the eosin step?

Was the slide adequately dehydrated prior to eosin staining?

Is the eosin stain contaminated with water?