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Guanine-Plus-Cytosine Content of DNA by the Thermal Melting Method

Specialty:

Clinical Laboratory

Objective:

ICD-10:

Description:

There is a linear relationship between the base composition of DNA as expressed in terms of the guanine plus cytosine bases and the denaturation (Tm, or thermal melting) temperature. E coli DNA serves as a reference material of known composition.

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