Agglutination in an affinity gel microtube can be graded based on the distribution of red cells relative to the gel.
Distribution of Red Blood Cells
Grade
only a layer of agglutinated cells on the top of the gel media
4+
agglutinated cells dispersed near the top of the gel media
3+
agglutinated cells dispersed through the entire length of the gel media
2+
agglutinated cells dispersed in the gel media near the bottom of the column
1+
only a button of unagglutinated cells in the bottom of the tube
negative
layer of agglutinated cells on the top of the gel media, AND a button of unagglutinated cells in the bottom of the tube
mixed field
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Specialty: Clinical Laboratory
