The fluorescence polarization assay involves adding a fluorescent dye to amniotic fluid which is then examined by fluorescence polarization. The results using the NBD-PC dye correlate with the amount of surfactant relative to albumin in the amniotic fluid. As the fetus matures, the value decreases. The clinical performance by this method is comparable to the L/S ratio.

To read more or access our algorithms and calculators, please log in or register.