Once leukemic cells have been determined to be negative with peroxidase, Sudan Black B and esterase stains, a further battery of stains can be used to characterize the leukemic process.
Panel of stains:
(1) terminal deoxynucleotidyl transferase (Tdt)
(2) myeloid antigen (CD11)
(3) toluidine blue (TB, stains granules in basophils and mast cells)
(4) platelet antigen (CD41)
(5) red blood cell antigen (RC 82.4)
Positive
Negative
Diagnosis
CD11
Tdt, TB, CD41, RC82.4
AML, undifferentiated
toluidine blue
Tdt, CD11, CD41, RC82.4
basophilic
Tdt and CD11
TB, CD41, RC82.4
biphenotypic (mixed lineage)
Tdt
CD11, TB, CD41, RC82.4
ALL
CD41
Tdt, CD11, TB, RC82.4
megakaryocytic
RC 82.4
Tdt, CD11, TB, CD41
erythroleukemia
none
Tdt, CD11, TB, CD41, RC82.4
undifferentiated leukemia
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