Description

Infusion of polymerized hemoglobin is one possible blood substitute that shows promise for use in situations where regular blood is not available. The different levels of free and red cell associated hemoglobin needs to be measured during therapy. Most hematology analyzers use a colorimetric assay to measure total hemoglobin in a blood sample.


 

Parameters:

(1) total blood hemoglobin by colorimetric method in g/dL

(2) hematocrit in percent

 

total hemoglobin in g/dL =

= (red blood cell associated hemoglobin) + (infused polymerized hemoglobin)

 

According to 03.06.02:

 

red blood cell associated hemoglobin in g/dL =

= ((percent hematocrit as a whole number) - 0.8) / 2.8

 

red blood cell associated hemoglobin in g/dL =

= (percent hematocrit as a whole number) / 2.941

 

where:

• There may be some individual variation from these relationships. In theory a pretransfusion blood sample could be used to derive a patient-specific factor correlating hemoglobin and hematocrit.

 

infused polymerized hemoglobin in g/dL =

= (total hemoglobin in g/dL) - (red blood cell hemoglobin estimated from hematocrit)

 


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