Description

The Kleihauer-Betke test is used to estimate the volume of a fetal-maternal hemorrhage. Fetal RBCs are rich in fetal hemoglobin, and fetal hemoglobin is resistant to acid elution. Thus staining for hemoglobin after an acid elution will stain RBCs with fetal hemoglobin, while RBCs with normal hemoglobins will be very faint.

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