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Estimating Protein and Nucleic Acid Concentration in Bacterial Extracts by UV Spectrophotometry

Specialty:

Clinical Laboratory

Objective:

ICD-10:

Description:

Proteins absorb UV light most strongly at 280 nm. Ultraviolet spectrophotometric methods of protein determination must take into account the strong absorbance of nucleic acids. The protein and nucleic acid concentrations in cell-free bacterial extracts can be estimated quickly and accurately by using the measured absorbances at 230 and 260 nm.

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