Equation of Pierelli et al for Estimating the Yield of CD34-Positive Cells During Autologous Stem Cell Apheresis

Method: continuous flow collection procedure

Parameters:

(1) number of CD34-positive cells per mL of peripheral blood

(2) mL of peripheral blood processed

(3) body weight in kilograms

CD34 positive cells per kg body weight collected =

= (number of CD34 positive cells per mL of peripheral blood) * 0.4 * (mL of peripheral blood processed) / (body weight)

where:

• 0.4 reflects the average component collection efficiency