Activities of blood transaminases, either aspartate (GOT) or alanine (GPT) aminotransferase, can serve as indications of a patient's vitamin B6 nutritional status. Enzymatic assays run before and after addition of pyridoxal phosphate can be used to generate an activity coefficient ratio. This represents the functional availability of erythrocyte vitamin B6 in its coenzyme form. Its value increases with vitamin B6 deficiency.

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