Description

Clostridium species are typically anaerobic but some are aerotolerant. Bacillus species are typically aerobc but some can grow under anaerobic conditions.


Culture selection: growth under anaerobic conditions and in 5-10% CO2

 

Assumption: pure culture of bacilli

 

Parameters:

(1) spore formation under anaerobic conditions

(2) spore formation under 5-10% CO2 atmosphere

(3) catalase reaction

 

Parameter

Aerotolerant Clostridium species

Facultatively Anaerobic Bacillus species

spore formation under anaerobic conditions

Yes

rare

spore formation under 5-10% CO2

rare

Yes

catalase

negative

positive

 


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