Clostridium species are typically anaerobic but some are aerotolerant. Bacillus species are typically aerobc but some can grow under anaerobic conditions.
Culture selection: growth under anaerobic conditions and in 5-10% CO2
Assumption: pure culture of bacilli
Parameters:
(1) spore formation under anaerobic conditions
(2) spore formation under 5-10% CO2 atmosphere
(3) catalase reaction
Parameter
Aerotolerant Clostridium species
Facultatively Anaerobic Bacillus species
spore formation under anaerobic conditions
Yes
rare
spore formation under 5-10% CO2
rare
Yes
catalase
negative
positive
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