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Determining the Proper Solvent Mix for High Pressure Liquid Chromatography (HPLC) to Provide a Specific Retention (Capacity) Factor

Specialty:

Clinical Laboratory

Objective:

ICD-10:

Description:

The mixture of solvents used in a HPLC column affects the retention of the solutes. If the retention time for a given mix of 2 solvents is known, then the proper mix of the same solvents to give a specific retention (capacity) factor can be calculated.

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