Immunohistochemical staining battery:
(1) GCDFP-15 (gross cystic disease fluid protein)
(2) ER (estrogen receptor)
(3) CEA (carcinoembryonic antigen)
(4) CK7 (cytokeratin 7)
|
Intensity of Staining
|
Points
|
|
none
|
0
|
|
mild
|
1
|
|
moderate
|
2
|
|
marked
|
3
|
immunohistochemical score =
= (percent of tumor staining as a whole number from 0 to 100) * (points for staining intensity)
where:
• minimum immunohistochemical score: 0
• maximum immunohistochemical score: 300
Decision Tree (Figure 1, page 287, as a Series of Rules)
Step 1: If the immunohistochemical score of the GCDFP-15 was > 10, then metastatic breast carcinoma.
Step 2: If the immunohistochemical score of the ER was > 95, then metastatic breast carcinoma.
Step 3: If the immunohistochemical score of the CEA was greater than the immunohistochemical score for CK7, then metastatic colon carcinoma.
Step 4: If all of the above are negative, then primary ovarian adenocarcinoma.
