Page et al reported the Cord Blood Apgar (CBA) to evaluate a banked cord blood sample. There are precryopreservation, post-thaw and composite scores. The authors are from Duke University, EMMES Corporation, Aldagen, Inc. and Cicada Biopharmaceutical Consulting.
Specimen selection: cord blood specimen, prior to cryopreservation
Parameters:
(1) colony forming units (CFUs) in 10^4
(2) total nucleated cells (TNCs) in 10^7
(3) CD34+ cells in 10^5
(4) mononuclear cell count (MNC) in 10^7
CFUs in 10^4/kg =
= (CFSs in 10^4) / (body weight)
TNCs in 10^7/kg =
= (CFSs in 10^4) / (body weight)
CD34+ cells in 10^5/kg =
= (CFSs in 10^4) / (body weight)
MNC in 10^7/kg =
= (MNC in 10^7) / (body weight)
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Parameters
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Findings
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Points
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CFUs in 10^4/kg
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> 7
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4
|
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3.51 to 7.0
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3
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1.51 to 3.5
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2
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<= 1.5
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1
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TNC in 10^7/kg
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> 9.4
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2
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5.71 to 9.4
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1.5
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1.31 to 5.7
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1
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<= 1.3
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0.5
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CD34+ in 10^5/kg
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> 3.2
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2
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1.81 to 3.2
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1.5
|
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1.01 to 1.8
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1
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<= 1
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0.5
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MNC in 10^7/kg
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> 3.6
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4
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|
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2.21 to 3.6
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3
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1.31 to 2.2
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2
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<= 1.3
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1
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postthaw score =
= SUM(points for all of the parameters)
composite score =
= (precryopreservation score) + (posththaw score)
Interpretation:
• minimum postthawscore: 3
• maximum postthawscore: 12
• minimum composite score: 5.25
• maximum composite score: 21
• The higher the score the better the neutrophil engraftment.
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PostthawScore
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Quartile
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>= 9.95
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first (highest)
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|
7.51 to 9.49
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second
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5.51 to 7.5
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third
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<= 5.5
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fourth
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Composite Score
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Quartile
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>= 17.25
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first (highest)
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13.51 to 17.74
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second
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9.76 to 13.50
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third
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<= 9.75
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fourth
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