Comparison of the difference between optical density peaks at 450 nm and 600 nm with the total protein content of the amniotic fluid could be correlated with the severity of fetal disease and the cord hemoglobin.
bilirubin-to-protein ratio =
= (OD450 - OD600) / (total protein in g/dL)
Ratio
Disease Severity
< 0.35
unaffected or mildly affected
0.35 to 0.55
moderately involved
> 0.55
severe involvement
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