Calculation of the Circulating Blood Volume Using Indocyanine Green (ICG) Pulse Dye Spectrophotometry


The circulating blood volume can be estimated by bolus injection of the dye indocyanine green (ICG) and monitoring blood levels over the next several minutes using pulse spectrophotometry. Pulse spectrophotometry is similar to pulse oximetry and allows the ratio of the arterial concentration of hemoglobin to ICG dye to be measured. From this ratio the concentration of ICG dye can be calculated. Based on the concentration of dye over time and the amount injected the blood volume can be calculated.

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