Peripheral blood progenitor cells (PBPC) are mobilized by the administration of colony stimulating factors. The response to the stimulation can be graded as good or poor based on certain criteria.
Method for mobilization: Administration of granulocyte colony stimulating factor (GCSF), either filgrastim or lenograstim.
Criteria for evaluating mobilization:
(1) number of CD34+ cells per µL
(2) number of CD34+ cells that are collected in a single apheresis procedure
(3) number of apheresis procedures required to achieve target dose
Criteria of Koenigsmann et al for a poor PBPC mobilization – one or more of the following:
(1) < 10 CD34+ cells per µL after stimulation
(2) < 2 * 10^6 CD34+ cells per kilogram collected during 1 leukapheresis procedure
Criteria of Ford et al for a good PBPC mobilization – both of the following:
(1) >= 65 CD34+ cells per µL after stimulation
(2) 1 apheresis collection to achieve target dose
For the implementation I will use 10 – 64 per µL as a fair mobilization.
Risk factors for poor mobilization:
(1) inadequate dose of GCSF
(2) for autologous donors, previous chemotherapy and/or irradiation
(3) more cells expressing CD11a (LFA-1) (Ford et al)
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Specialty: Clinical Laboratory, Hematology Oncology