The size of gel chromatography column can be determined by the separation needs.
Goal
Column Size in mL
separation to 20 g
100 * (separation sample size ini grams)
separation from 21 to 250 g
80 * (separation sample size in grams)
separation from 250 to 300 g
filtration to 250 grams
40 * (filtration sample size in grams)
filtration from 250 to 300 g
66 * (filtration sample size in grams
upper limit of fraction size in mL =
= (column size in mL) / 20
For a column above 2,000 mL the lower limit to the fraction size may be 25 to 33% less.
A more complex equation for columns 4,000 to 20,00 mL
fraction size in mL =
= (-0.01074 * ((column size) / 100)^2) + (5.969 * (column size) / 100) - 13.63
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Specialty: Clinical Laboratory
