Moreau et al developed a scoring system for the immunocytochemical staining pattern of B lymphocytes. This can help distinguish chronic lymphocytic leukemia (CLL) from other B-cell lymphoproliferative disorders.
Patients selection: B-cell lymphoproliferative disorder
Immunostains performed:
(1) monoclonal surface immunoglobulins
(2) CD5
(3) CD23
(4) FMC7
(5) CD22 or CD79b
|
Marker |
Staining Reaction |
Points |
|
surface immunoglobulins |
strong |
0 |
|
|
weak |
1 |
|
CD5 |
negative |
0 |
|
|
positive |
1 |
|
CD23 |
negative |
0 |
|
|
positive |
1 |
|
FMC7 |
positive |
0 |
|
|
negative |
1 |
|
CD22 or CD79b |
weak |
0 |
|
|
strong |
1 |
total score =
= SUM(points for all 5 immunostains)
Interpretation:
• minimum score: 0
• maximum score: 5
|
Total Score |
Interpretation |
|
0 to 2 |
other B-cell |
|
3 |
indeterminate |
|
4 or 5 |
CLL |
Specialty: Hematology Oncology, Clinical Laboratory
