Nucleic acids in solution can be quantitated using absorbance by ultraviolet (UV) spectrophotometry.
Testing instrumentation:
(1) ultraviolet spectrophotometer
(2) cuvettes with 1 cm light path
Nucleic Acid
Concentration Giving an Absorbance of 1.0 at 260 nm
double stranded DNA
50 µg/mL
single stranded DNA
33 µg/mL
single stranded RNA
40 µg/mL
concentration of nucleic acid in µg/mL =
= (concentration giving absorbance at 1.0) * (absorbance of solution at 260 nm)
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Specialty: Clinical Laboratory
