Description

A specimen that is pipetted after one with a high concentration of an analyte may show a falsely high reading, a finding termed carryover. This may result from inadequate washing of the pipette, the pipette material, the sample medium, and other factors. The degree of carryover for a pipette can be calculated using samples with known low and high concentrations of an analyte.


 

Protocol:

(1) Test a specimen with a low concentration of the analyte in triplicate.

(2) Test a specimen with a very high concentration of the same analyte.

(3) Test the specimen with a low concentration again.

 

percent carryover =

= ((result from step 3) – (mean for the 3 readings in step 1)) / (reading from step 2) * 100%

 


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