Description

As pregnancy progresses, the amniotic fluid becomes more turbid. This can be semiquantitated by measuring its absorbance at 650 nm. It correlates with the increase in lamellar bodies, which serve to scatter light and thus make the fluid appear turbid.


 

Specimen: Amniotic fluid, protected from light

 

Testing: Spectrophotometric absorbance of amniotic fluid at 650 nm.

 

Interpretation:

• optical absorbance >= 0.1 indicates maturity

 

Interferences:

(1) heme pigment

(2) porphyrins

(3) bilirubin

 

Limitations:

• Polyhydramnios results in false negative values due to dilutional effect.

 


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