Hans et al developed an algorithm for using immunostains to subdivide patients with diffuse large B-cell lymphoma (DLBCL) into molecular subtypes. The authors are from multiple institutions in North America and Europe.
Subtypes of DLBCL:
(1) germinal center B-cell-like (GCB)
(2) activated B-cell like (ABC)
(3) Type 3
Immunostains:
(1) CD10
(2) BCL6: (B cell lymphoma 6)
(3) MUM1 (multiple myeloma oncogene 1
The types determined by immunostains were compared with results of gene expression profiling (GEP).
CD10
BCL6
MUM1
Type
>= 30%
NA
NA
GCB
< 30%
>= 30%
>=30%
non-GCB
< 30%
>= 30%
< 30%
GCB
< 30%
< 30%
NA
non-GCB
where:
• The non-GCB type consisted of ABC and Type 3.
Performance:
• The method (clone, source, antigen retrieval, instrument, analysis) is necessary to get the same results.
To read more or access our algorithms and calculators, please log in or register.
