Polyagglutinable red blood cells contain an inherited or acquired antigen that reacts with antibodies present in the serum of most adults. These cells may be difficult to antigen type if human antisera is used, and can cause problems when crossmatching blood for transfusion. The antigen involved can be identified by testing the polyagglutinable cells against plant lectins.
Common polyagglutinable antigens:
(1) T
(2) Tk
(3) Th
(4) Tx
(5) Tn
(6) Cad
Lectins:
(1) Arachis hypogaea (peanut)
(2) Dolichos biflorus
(3) Glycine max (soya, soy beans)
(4) Griffonia simplicifolia II
(5) Vicia cretica
(6) Salvia sclarea
(7) Salvia horminum
(8) Leonurus cardiaca
(9) Polybrene
|
Lectin |
T |
Tk |
Th |
|
Arachis hypogaea |
positive |
positive |
positive |
|
Dolichos biflorus |
0 |
0 |
0 |
|
Glycine max |
positive |
0 |
0 |
|
Griffona simplicifolia II |
0 |
positive |
0 |
|
Vicia cretica |
positive |
0 |
positive |
|
Salvia sclarea |
0 |
0 |
0 |
|
Salvia horminum |
0 |
0 |
0 |
|
Leonurus cardiaca |
weak |
0 |
0 |
|
Polybrene |
0 |
positive |
positive |
where:
• Dolichos biflorus agglutinates A1 antigen, so polyagglutinable typing can only be done with type O or B cells (and probably A2 cells).
|
Lectin |
Tx |
Tn |
Cad |
|
Arachis hypogaea |
positive |
0 |
0 |
|
Dolichos biflorus |
0 |
positive |
positive |
|
Glycine max |
0 |
positive |
variable |
|
Griffona simplicifolia II |
0 |
0 |
0 |
|
Vicia cretica |
0 |
0 |
0 |
|
Salvia sclarea |
0 |
positive |
0 |
|
Salvia horminum |
0 |
positive |
positive |
|
Leonurus cardiaca |
0 |
0 |
positive |
|
Polybrene |
positive |
0 |
positive |
where:
• Weak Cad antigenic expression may have negative reactions with Glycine max.
Specialty: Clinical Laboratory
