Variation in absorbance ratios at 260 nm to 230 nm (A260 / A230) for different nucleic acids
(1) RNA for torula yeast: 2.42
(2) DNA bacterial species: 2.20 - 2.43
(3) tRNA from E.coli : 2.20 - 2.35
(4) rRNA bacterial: 2.5
In most situations (assumes nucleic acid A260 / A230 is about 2.42):
protein concentration in µg/mL =
= (183 * (absorbance at 230 nm)) - (75.8 * (absorbance at 260 nm))
If tRNA is an important nucleic acid component (assumes nucleic acid A260 / A230 is about 2.25):
protein concentration in µg/mL =
= (184 * (absorbance at 230 nm)) - (81.7 * (absorbance at 260 nm))
nucleic acid concentration in µg/mL =
= (49.1 * (absorbance at 230 nm)) - (3.48 * (absorbance at 260 nm))
