The CH50 (total hemolytic complement activity) in body fluid is typically expressed as a ratio to the serum level together with a correction for protein.
Specimen collection: Both the body fluid and serum samples should be drawn at the same time.
Parameters:
(1) CH50 activity in the body fluid in U/mL
(2) CH50 activity in serum in U/mL
(3) protein concentration in the body fluid in g/dL
(4) protein concentration in serum in g/dL
ratio of CH50 activity in body fluid to serum as a percent =
= ((CH50 activity in body fluid) / (CH50 activity in serum)) / ((protein concentration in the body fluid) / (protein concentration in serum)) * 100 =
= (CH50 activity in body fluid) * (protein concentration in serum) * 100 / ((CH50 activity in serum) * (protein concentration in the body fluid))
Body Fluid
Normal Ratio
synovial fluid
approximately 70%
pleural fluid
approximately 70%
CSF
approximately 70%
synovial fluid
>= 33%
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