A variety of staining patterns can be seen on indirect immunofluorescent antinuclear antibody testing. The pattern seen correlates with the underlying disease, which can be diagnosed by performing additional tests.
|
Immunofluorescent Pattern |
Disease |
|
nuclear rim (peripheral) |
SLE |
|
|
drug-induced lupus |
|
|
other connective tissue diseases |
|
homogeneous (diffuse) |
SLE |
|
|
drug-induced lupus |
|
|
other connective tissue diseases |
|
nuclear rim and homogeneous |
SLE |
|
|
drug-induced lupus |
|
|
other connective tissue diseases |
|
speckled (extractable nuclear antigen) |
rheumatoid arthritis |
|
|
SLE |
|
|
mixed connective tissue disease |
|
|
Sjogren's syndrome |
|
atypical speckled |
scleroderma |
|
nucleolar |
peripheral systemic sclerosis |
|
|
Sjogren's |
|
|
SLE |
|
centromere |
CREST |
|
|
scleroderma |
|
Disease |
Additional Tests |
|
systemic lupus erythematosus |
anti-double stranded DNA |
|
|
anti- single stranded DNA |
|
|
anticardiolipin |
|
|
anti-Sm (Smith), with speckled pattern |
|
drug-induced lupus |
anti-histone |
|
CREST |
Scl-70 |
|
|
anti-RNP |
|
scleroderma |
SS-A/Ro |
|
|
SS-B/La |
|
|
Scl-70 |
|
peripheral systemic sclerosis |
anti-RNP |
|
rheumatoid arthritis |
rheumatoid factor |
|
mixed connective tissue disease |
anti-RNP |
|
Sjogren's syndrome |
SS-A/Ro |
|
|
SS-B/La |
|
|
antihistone |
|
other connective tissue disease |
|
Purpose: To use the pattern of immunofluorescence shown by antinuclear antibodies to indicate a patient's underlying immune disorder.
Specialty: Immunology/Rheumatology, Clinical Laboratory
Objective: clinical diagnosis, including family history for genetics, imaging studies, laboratory tests, disease progression
ICD-10: K75.4, L93, M32.0, M33.0, M33.1, M33.2, M34, M35.0, M35.1,
