Method for mobilization: Administration of granulocyte colony stimulating factor (GCSF), either filgrastim or lenograstim.
Criteria for evaluating mobilization:
(1) number of CD34+ cells per µL
(2) number of CD34+ cells that are collected in a single apheresis procedure
(3) number of apheresis procedures required to achieve target dose
Criteria of Koenigsmann et al for a poor PBPC mobilization – one or more of the following:
(1) < 10 CD34+ cells per µL after stimulation
(2) < 2 * 10^6 CD34+ cells per kilogram collected during 1 leukapheresis procedure
Criteria of Ford et al for a good PBPC mobilization – both of the following:
(1) >= 65 CD34+ cells per µL after stimulation
(2) 1 apheresis collection to achieve target dose
For the implementation I will use 10 – 64 per µL as a fair mobilization.
Risk factors for poor mobilization:
(1) inadequate dose of GCSF
(2) for autologous donors, previous chemotherapy and/or irradiation
(3) more cells expressing CD11a (LFA-1) (Ford et al)
